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Related Experiment Videos

Enterococcus faecalis adhesin, Ace, mediates attachment to particulate dentin.

William J Kowalski, Edward L Kasper, John F Hatton

    Journal of Endodontics
    |June 24, 2006
    PubMed
    Summary

    Enterococcus faecalis (E. faecalis) adhesion to dentin is mediated by the collagen-binding protein Ace. Enhanced Ace expression increases bacterial binding, which can be inhibited by collagen and anti-Ace antibodies.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Biomaterials Science

    Background:

    • Enterococcus faecalis (E. faecalis) is a common cause of persistent infections in root canals.
    • Bacterial adhesion to dentin is a critical step in the pathogenesis of endodontic infections.
    • The collagen-binding protein Ace on E. faecalis has been implicated in dentin colonization.

    Purpose of the Study:

    • To investigate the role of the collagen-binding protein Ace in E. faecalis adhesion to particulate dentin.
    • To quantify the effect of Ace expression levels on bacterial-dentin interactions.
    • To explore potential inhibition strategies for E. faecalis adhesion.

    Main Methods:

    • Development of an enzyme-linked immunosorbent assay (ELISA) to measure E. faecalis binding to dentin.

    Related Experiment Videos

  • Culturing E. faecalis strains with varying Ace expression (OG1RF Ace+ at 46°C, OG1RF Ace+ at 37°C, TX5256 Ace- at 37°C).
  • Assessing bacterial adhesion over time (0-360 minutes) and evaluating inhibition by type I collagen and anti-Ace IgG.

    • Main Results:

    • E. faecalis grown at 46°C (enhanced Ace expression) showed significantly higher adhesion to dentin compared to controls at most time points (p < 0.001).
    • Maximum binding of OG1RF (Ace+) grown at 46°C occurred at 120 minutes (17.4% of positive control).
    • Type I collagen and anti-Ace IgG significantly inhibited the adhesion of E. faecalis (Ace+) grown at 46°C to dentin.

    Conclusions:

    • The collagen-binding protein Ace plays a significant role in the adhesion of E. faecalis to dentin.
    • Increased Ace expression enhances bacterial adherence, suggesting its importance in dentin colonization.
    • Targeting Ace or its interaction with collagen may represent a viable strategy to prevent E. faecalis colonization in endodontic infections.