Related Experiment Video
Updated: Aug 7, 2026

Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
Inhibitory effects of cisplatin and its conjugate with glutathione on blood platelet activity
1Department of General Biochemistry, Institute of Biochemistry, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Abstract:
Cisplatin (cis -diamminedichloroplatinum II, CDDP) is a widely used chemotherapeutic agent; however, its haematological toxicity may become an important dose-limiting factor. The aim of the present study was to evaluate the effects of cisplatin and its metabolite with glutathione (GS-Pt conjugate) on thrombin-induced platelet aggregation, the secretory process and the arachidonate pathway in vitro. Pre-treatment of platelets with GS-Pt conjugate (60 min, 20 microM) but not with cisplatin alone, caused inhibition of thrombin-induced platelet aggregation. GS-Pt conjugate had also a strong inhibitory effect on the release of proteins and adenine nucleotides from platelet granules. After pre-incubation of platelets with cisplatin and its conjugate, we observed in thrombin-activated platelets, a decreased amount of malonyldialdehyde (a marker of thromboxane A(2) synthesis). We suggest that arachidonate metabolism plays an important role in determining the cytotoxicity of cisplatin.
Related Concept Videos
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Pharmacokinetics: Drug–Drug Interactions
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow
Drugs that Destabilize Microtubules

