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Validation of a solid-phase extraction high-performance liquid chromatographic assay for doxazosin
G P Jackman1, F Colagrande, W J Louis
1Department of Clinical Pharmacology and Therapeutics, Austin Hospital, Heidelberg, Victoria, Australia.
Journal of Chromatography
|May 3, 1991
Summary
This study presents a new method for measuring doxazosin in plasma using high-performance liquid chromatography and fluorescence detection. The validated technique is sensitive and efficient, suitable for clinical trials.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Accurate quantification of pharmaceuticals in biological matrices is crucial for clinical pharmacology.
- Doxazosin is an alpha-blocker used to treat hypertension and benign prostatic hyperplasia.
Purpose of the Study:
- To develop and validate a sensitive method for determining doxazosin in human plasma.
- To apply the method to analyze samples from clinical trials.
Main Methods:
- High-performance liquid chromatography (HPLC) with fluorescence detection.
- Propranolol as an internal standard.
- C18 reversed-phase extraction for sample cleanup.
Main Results:
- The method achieves a determination limit of 1 ng/ml in plasma.
- C18 extraction columns demonstrated good reusability.
- Successful application to clinical trial samples.
Conclusions:
- A reliable and reusable HPLC-fluorescence method for doxazosin determination in plasma was established.
- The method's sensitivity and efficiency make it suitable for pharmacokinetic and clinical studies.