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Related Experiment Videos

Urine collection from disposable nappies.

T Ahmad1, D Vickers, S Campbell

  • 1Department of Child Health, Royal Victoria Infirmary, Newcastle upon Tyne, UK.

Lancet (London, England)
|September 14, 1991
PubMed
Summary
This summary is machine-generated.

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Collecting urine from young children using disposable nappies is a viable alternative to standard urine bags. This method is effective for bacterial culture, microscopy, and biochemical analysis, with lower contamination rates.

Area of Science:

  • Pediatrics
  • Clinical Chemistry
  • Microbiology

Background:

  • Urine sample collection in infants and young children is challenging.
  • Standard methods like urine bags can be invasive or lead to contamination.
  • Alternative, non-invasive methods are needed for accurate urinalysis.

Purpose of the Study:

  • To evaluate the efficacy of collecting urine from disposable nappies for various analyses.
  • To compare nappy-based urine collection with standard urine bags in young children.
  • To assess the reliability of nappy-collected urine for microscopy, culture, and biochemical testing.

Main Methods:

  • Urine samples were collected from 45 children (1-23 months) using sterile adhesive bags and by extraction from wet disposable nappies.

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  • Nappy collection involved compressing fibres, avoiding highly absorbent brands with gel beads.
  • Microscopy, bacterial culture, and biochemical analyses (sodium, potassium, urea, creatinine, osmolality, calcium, phosphate) were performed on both sample types.

    • Main Results:

    • Urine extraction from nappies is feasible, especially when avoiding gel bead brands.
    • Microscopy showed reduced cell counts from nappies, but bacterial counts were comparable.
    • Culture identified urinary tract infections similarly in both methods; nappy collection had lower contamination rates (10/39 vs 17/39).
    • Biochemical analysis showed high correlation for most analytes (sodium, potassium, urea, creatinine, osmolality) between fresh urine and nappy samples soaked for 3 hours, with greater variation for calcium and phosphate.

    Conclusions:

    • Extraction of urine from disposable nappies is an inexpensive, rapid, and simple method for young children.
    • This technique is suitable for bacterial culture, microscopy, and biochemical analysis.
    • Nappy collection offers a less contaminating and practical alternative to urine bags for pediatric urinalysis.