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Fluorogenic nanocrystals for highly sensitive detection of C-reactive protein
K-K Sin1, C P-Y Chan, W-M Leung
1Department of Chemistry, Kowloon, The Hong Kong University of Science &Technology, Clear Water Bay, Kowloon.
IEE Proceedings. Nanobiotechnology
|June 27, 2006
Summary
This study introduces a novel fluorescence immunoassay for detecting C-reactive protein (CRP) using fluorescein diacetate (FDA) nanocrystals. The assay demonstrates high precision and a low detection limit, making it a promising tool for diagnosing inflammation and heart conditions.
Area of Science:
- Biochemistry
- Nanotechnology
- Immunodiagnostics
Background:
- C-reactive protein (CRP) is a key biomarker for acute inflammation and heart failure.
- Existing diagnostic methods for CRP may lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop and validate a solid-phase sandwich fluorescence immunoassay for CRP detection.
- To utilize novel fluorescein diacetate (FDA) nanocrystals for enhanced assay performance.
Main Methods:
- Conjugation of monoclonal antibodies to DSPE-PEG-modified FDA nanocrystals.
- Development of a fluorescence immunoassay protocol for CRP detection in human serum.
- Validation against a commercial turbidimetric immunoassay using Bland-Altman analysis.
Main Results:
- Achieved a low limit of detection (1.10 microg l(-1)) and high precision (CV = 2.72-9.48%).
- Demonstrated good correlation with a commercial CRP assay in patient and healthy donor serum samples.
- Bland-Altman analysis showed a mean difference of 0.36+/-1.46 mg l(-1) between methods.
Conclusions:
- Organic fluorogenic FDA nanocrystals are effective for CRP detection.
- The developed immunoassay offers a sensitive and precise method for clinical diagnostics.
- This approach holds potential for early detection of inflammation and heart-related conditions.