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Rat peritoneal macrophage procoagulant and fibrinolytic activities. An expression of the local inflammatory response
A M Freyria1, J Paul, J Belleville
1INSERM U 37, Bron, France.
Abstract:
1. Lysates of rat (Rattus norvegicus, Wistar strain) peritoneal macrophages selected by adherence were analysed for procoagulant activity (PCA) and plasminogen activator activity (PAA). 2. PCA is expressed following in vitro stimulation by lipopolysaccharide and in vivo (respectively 118 +/- 19.1 and 147.2 +/- 45.2 mUnits/10(6) M phi) very early after the intraperitoneal injection of thioglycollate. 3. PAA present in 24 hr thioglycollate stimulated cells (79.5 +/- 26.1 mI.U./10(6) M phi), disappears in a time dependent fashion after in vitro lipopolysaccharide stimulation. 4. Our findings indicate that rat peritoneal macrophages regulate their coagulolytic activities in a specific manner. 5. Rat peritoneal cavity may be proposed as a model for the study of inflammatory reaction with PCA and PAA as its indexes.
Insights
Rat peritoneal macrophages exhibit specific regulation of procoagulant activity (PCA) and plasminogen activator activity (PAA). These activities serve as valuable indexes for studying inflammatory reactions in the rat peritoneal cavity model.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Peritoneal macrophages play a crucial role in inflammatory responses.
- Understanding the coagulolytic activities of macrophages is essential for studying inflammation.
Purpose of the Study:
- To analyze the procoagulant activity (PCA) and plasminogen activator activity (PAA) in rat peritoneal macrophages.
- To investigate the regulation of these activities upon stimulation.
Main Methods:
- Lysates of rat peritoneal macrophages were analyzed.
- Cells were stimulated in vitro with lipopolysaccharide and in vivo with thioglycollate.
Main Results:
- Procoagulant activity (PCA) was expressed following both in vitro and in vivo stimulation.
- Plasminogen activator activity (PAA) was present in thioglycollate-stimulated cells but decreased with lipopolysaccharide stimulation.
Conclusions:
- Rat peritoneal macrophages demonstrate specific regulation of their coagulolytic activities.
- The rat peritoneal cavity can be utilized as a model to study inflammatory reactions using PCA and PAA as indexes.