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SFTG international collaborative study on in vitro micronucleus test III. Using CHO cells.
Marilyn J Aardema1, Ronald D Snyder, Carol Spicer
1Procter & Gamble Co., Miami Valley Laboratories, Cincinnati, OH 45253-8707, USA.
Mutation Research
|June 27, 2006
Summary
This international study validated the in vitro micronucleus assay using Chinese hamster ovary cells. The assay effectively detected genotoxic chemicals, confirming its suitability for routine mutagenicity testing.
Area of Science:
- Toxicology
- Genetics
- Cell Biology
Background:
- The in vitro micronucleus assay is a key method for detecting chromosomal damage.
- Standardization of protocols is crucial for reliable genotoxicity testing.
- Chinese hamster ovary (CHO) cells are widely used in toxicological studies.
Purpose of the Study:
- To evaluate the suitability of the in vitro micronucleus assay using CHO cells.
- To compare different testing protocols for optimizing the assay.
- To assess the reliability of CHO cells in detecting genotoxic effects.
Main Methods:
- An international collaborative study involving multiple laboratories.
- Evaluation of chemicals including mannitol, bleomycin, cytosine arabinoside, urethane, and diethylstilboestrol.
- Application of various protocols: short/long exposures, recovery times, and use of cytochalasin B.
Main Results:
- Mannitol and urethane showed no genotoxic effects.
- Bleomycin, cytosine arabinoside, and diethylstilboestrol induced a dose-dependent increase in micronucleated cells.
- The assay detected these genotoxic effects across different treatment and recovery conditions, with or without cytochalasin B.
Conclusions:
- Chinese hamster ovary cells are suitable for the in vitro micronucleus assay.
- The assay demonstrated consistent detection of genotoxic agents under various conditions.
- Results support the establishment of a standardized protocol for routine genotoxicity testing using CHO cells.