Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA methylation mapping by tag-modified bisulfite genomic sequencing.

Weiguo Han1, Stephane Cauchi, James G Herman

  • 1Laboratory of Human Toxicology and Molecular Epidemiology, Wadsworth Center, NYS Department of Health, Albany, NY 12201, USA.

Analytical Biochemistry
|June 27, 2006
PubMed
Summary

A new tag-modified bisulfite genomic sequencing (tBGS) method simplifies DNA methylation analysis. This technique allows for large-scale genomic DNA scanning, aiding comprehensive methylation mapping studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leveraging Commercially Available Protein Assays as Biomarkers for Lung Cancer.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same author

Defining the acute BTK-loss transcriptional program in CLL using a BTK degrader.

Leukemia·2026
Same author

Role of P450 enzymes of the Cyp2abfgs gene subfamilies in tobacco smoke-induced lung tumorigenesis in mice.

Carcinogenesis·2026
Same author

3,3'-Dichlorobiphenyl (PCB 11) alters the hepatic expression of cytochrome P450 enzymes in the liver of mouse dams exposed orally during pregnancy and lactation.

Archives of toxicology·2025
Same author

Lnc5q21.2, a novel long intergenic RNA, sensitizes colorectal cancer cells to ATR inhibitor by activating Wnt pathway.

Journal of translational internal medicine·2025
Same author

Protection of infant mice against pertussis, tuberculosis and influenza by co-administration of nasal pertussis vaccine candidate BPZE1 and BCG.

iScience·2025

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation is crucial for gene regulation.
  • Accurate and scalable methods for DNA methylation analysis are needed.
  • Conventional methods can be laborious and time-consuming.

Purpose of the Study:

  • To develop a simplified method for evaluating DNA methylation sites at kilobase scale.
  • To assess the utility of the new method for exploring CpG methylation differences across tissues and cell types.
  • To investigate gene promoter methylation patterns for CYP1B1 and GSTP1.

Main Methods:

  • Developed tag-modified bisulfite genomic sequencing (tBGS) using direct cycle sequencing of PCR products.
  • Applied tBGS without conventional DNA fragment cloning.

Related Experiment Videos

  • Utilized GC-tagged primers for a second-round PCR amplification of bisulfite-modified DNA.

    • Main Results:

    • tBGS results showed high correlation with conventional bisulfite genomic sequencing (R=0.935, p=0.002).
    • Identified predominantly conserved CpG methylation maps for CYP1B1 and GSTP1 promoters across human tissues and individuals.
    • Observed distinct methylation patterns in the GSTP1 promoter between normal (NHBE) and malignant (A549) lung cells, correlating with gene expression differences.

    Conclusions:

    • The tBGS method offers a simplified approach for detailed methylation scanning of large genomic regions.
    • This technique facilitates more ambitious genomic methylation mapping studies.
    • tBGS is a valuable tool for understanding epigenetic variations in health and disease.