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Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 25, 2011
The (ADP-ribosyl)ation reaction in thermophilic bacteria
Maria Rosaria Faraone-Mennella1, Anna De Maio, Anna Petrella
1Dipartimento di Biologia Strutturale e Funzionale, Facolta' di Scienze M.F.N., Universita Federico II, Via Cynthia, 80126 Napoli, Italy. faraone@unina.it
Abstract:
Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cells at different growth phases, and evidence of an active system was obtained in all analyzed microorganisms, with comparable enzymatic levels. Immunochemical analyses with polyclonal antibodies against both eukaryotic anti-(ADP-ribose) transferase and anti-poly(ADP-ribose) polymerase revealed, for all tested organisms, an immunosignal localized in the range of molecular masses between 43-53 kD. Several proteins of various molecular masses were found as ADP-ribose acceptors. Reaction product analyses showed mono(ADP-ribose) to be the only synthesized compound.
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