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Updated: Aug 7, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Analysis of Mammalian sperm-egg membrane interactions during in vitro fertilization
Genevieve B Wortzman1, Allison J Gardner, Janice P Evans
1Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins University of Bloomberg School of Public Health, Baltimore, MD, USA.
Abstract:
The interactions between egg and sperm are among the most fascinating in cell biology. These interactions include cell-cell adhesion and then membrane fusion between the two gametes. This chapter details the experimental methods used to for gamete culture and in vitro fertilization using mouse sperm and eggs.
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