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Related Experiment Video

Updated: May 20, 2026

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
10:45

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

Published on: October 13, 2011

Collagen gel contraction assay.

Peter Ngo1, Punitha Ramalingam, Jonathan A Phillips

  • 1Fellow in Pediatric Gastroenterology, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 27, 2006
PubMed
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Chronic inflammation stiffens the intestine, impairing motility. The collagen lattice assay models tissue contraction, revealing how cells and matrix interact during inflammation and healing.

Area of Science:

  • Gastroenterology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Intestinal motility is crucial for normal function, regulated by intrinsic innervation.
  • Chronic inflammatory diseases like Crohn's disease cause intestinal stiffening and fibrosis, reducing motility.
  • Non-muscle tissue contraction is vital in inflammation, wound healing, and remodeling, but mechanisms are unclear.

Purpose of the Study:

  • To investigate the physiological and pathological mechanisms of tissue contraction in the gastrointestinal tract.
  • To explore the interaction between cellular components and the extracellular matrix in tissue contraction.
  • To utilize a novel model system for studying tissue contraction dynamics.

Main Methods:

  • Development and application of the collagen lattice contraction assay.

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Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
06:02

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

Related Experiment Videos

Last Updated: May 20, 2026

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
10:45

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

Published on: October 13, 2011

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
06:02

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

  • Utilizing cell-populated collagen hydrogels to model tissue contraction.
  • Investigating the influence of specific agonists on matrix contraction rates and extent.

    • Main Results:

    • The collagen lattice assay demonstrates predictable and consistent contraction of cell-populated collagen hydrogels over time.
    • This model allows for the study of cellular and extracellular matrix interactions in tissue contraction.
    • The assay facilitates the investigation of agonist effects on the dynamics of matrix contraction.

    Conclusions:

    • The collagen lattice contraction assay is a valuable model for studying tissue contraction.
    • Understanding cell-matrix interactions is key to defining mechanisms of tissue contraction in health and disease.
    • This model system offers insights into pathological processes like fibrosis and remodeling in chronic inflammatory conditions.