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Updated: Aug 5, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Development of restricted-access media supports and their application to the direct analysis of biological fluid
N M Cassiano1, V V Lima, R V Oliveira
1Departamento de Química, Universidade Federal de São Carlos, Cx. Postal 676, São Carlos, SP, 13565-905, Brazil.
Abstract:
A quick overview of published methods for analyzing compounds in complex biological samples reveals that the most difficult step is the clean-up or extraction of a required compound from the matrix. The strategy required to analyze exogenous compounds in biological fluids depends greatly upon the nature of the compound and upon the biomatrix. Coupled-column separation using restricted-access media as the first dimension in order to exclude macromolecules and retain micromolecules has been successfully used for a number of biological fluids. This paper presents the history of the development of restricted-access media supports and of their application to the direct injection of biological fluid samples in high-performance liquid chromatography.
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