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Related Experiment Videos

Dextran derivatives modulate collagen matrix organization in dermal equivalent.

Laetitia Frank1, Corinne Lebreton-Decoster, Gaston Godeau

  • 1THERAPOL S.A., 63 rue de Strasbourg, 93200 Saint Denis, France. laetitia_frank@yahoo.fr

Journal of Biomaterials Science. Polymer Edition
|June 28, 2006
PubMed
Summary

Dextran derivatives with sulfate groups promote fibroblast proliferation and enhance dermal wound healing by accelerating collagen organization and increasing matrix metalloproteinase-2. This bioactive polymer may aid in treating hypertrophic scars.

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Area of Science:

  • Biomaterials Science
  • Dermatology
  • Regenerative Medicine

Background:

  • Dextran derivatives show potential in protecting growth factors crucial for wound healing, like transforming growth factor-beta1 (TGF-beta1) and fibroblast growth factor-2 (FGF-2).
  • Understanding the impact of dextran modifications on fibroblast behavior is key to developing effective wound healing therapies.

Purpose of the Study:

  • To investigate the effects of various dextran derivatives on human dermal fibroblasts in vitro.
  • To evaluate the efficacy of a specific dextran derivative (LS21 DMCBSu) in promoting dermal wound healing in a collagenous matrix model.

Main Methods:

  • Culturing human dermal fibroblasts and treating them with different dextran derivatives (methylcarboxylate, benzylamide, sulphate substituted).
  • Utilizing a dermal equivalent model with fibroblasts in collagenous matrices, treated with LS21 DMCBSu, TGF-beta1, or FGF-2.

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  • Employing histology, immunohistochemistry, zymography, and Western blot to analyze collagen organization, collagen type-III expression, myofibroblast apoptosis, and matrix metalloproteinase-2 (MMP-2) secretion.

    • Main Results:

    • Sulphate groups on dextran derivatives were found to be essential for fibroblast proliferation.
    • The selected dextran derivative (LS21 DMCBSu) accelerated collagen matrix organization and stimulated human type-III collagen expression in dermal equivalents.
    • The bioactive polymer induced myofibroblast apoptosis and significantly increased the secretion of both zymogen and active matrix metalloproteinase-2 (MMP-2).

    Conclusions:

    • Dextran derivatives, particularly those with sulphate groups, positively influence fibroblast proliferation and extracellular matrix remodeling.
    • The specific dextran derivative LS21 DMCBSu demonstrates significant potential for enhancing dermal wound healing by modulating collagen synthesis and degradation.
    • This bioactive polymer's ability to induce myofibroblast apoptosis and increase MMP-2 levels suggests therapeutic benefits for wound healing and hypertrophic scar treatment.