Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Abnormal spermatogenesis in mice unable to synthesize ascorbic acid.

Futoshi Yazama1, Kazuko Furuta, Miwa Fujimoto

  • 1epartment of Life Sciences, Laboratory of Cell Biology and Morphology, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara City, Hiroshima 727-0023, Japan. fyzma@pu-hiroshima.ac.jp

Anatomical Science International
|June 28, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chaetoceros gracilis, a marine microalga, attenuates hepatic lipid accumulation in SDT fatty rats, a severe genetic MASLD model.

Journal of oleo science·2026
Same author

Initial Experience With Endoscopic Ultrasound-Guided Gallbladder Drainage Using Lumen-apposing Metal Stents in Extremely Elderly and Frail Patients.

DEN open·2026
Same author

A Case of Malan Syndrome With Pulmonary Artery Dilatation due to a Novel Frameshift Variant in Exon 2 of the <i>NFIX</i> Gene.

Case reports in genetics·2026
Same author

Antemortem diagnosis combining computed tomography and air sac fluid aspiration for aspergillosis: a case report in a gentoo penguin (Pygoscelis papua).

The Journal of veterinary medical science·2026
Same author

Lobular dissecting hepatitis-like chronic hepatitis with bacterial chronic cholangitis in a ferret (Mustela putorius furo).

The Journal of veterinary medical science·2026
Same author

Percutaneous endoscopic necrosectomy using a novel slim gastroscope with a large working channel for pancreatic walled-off necrosis.

Endoscopy·2026

Dietary vitamin C (ascorbic acid) deficiency in mice leads to male infertility by causing spermatocyte apoptosis. This is linked to the disappearance of heat-shock protein 70, crucial for sperm development.

Area of Science:

  • Reproductive Biology
  • Biochemistry
  • Toxicology

Background:

  • Male infertility pathogenesis is unclear, despite links to environmental toxicants.
  • Ascorbic acid (vitamin C) is essential, but its role in male reproduction needs further study.
  • Gulo-/- mutant mice lack ascorbic acid synthesis, serving as a model for vitamin C deficiency.

Purpose of the Study:

  • To investigate the impact of dietary vitamin C deficiency on spermatogenesis in Gulo-/- mice.
  • To explore the relationship between vitamin C levels, spermatocyte apoptosis, and heat-shock protein 70 expression in testes.

Main Methods:

  • Utilized Gulo-/- mutant mice with and without vitamin C supplementation.
  • Conducted morphological and biochemical analyses of testes from vitamin C-deficient and sufficient mice.

Related Experiment Videos

  • Employed two-dimensional electrophoresis to analyze protein expression, specifically heat-shock protein 70.

    • Main Results:

    • Vitamin C deficiency induced frequent apoptosis of spermatocytes in 20-day-old Gulo-/- mice.
    • Heat-shock protein 70 was specifically absent in the testes of vitamin C-deficient mice.
    • Vitamin C supplementation ameliorated the deficiency and supported mutant mouse growth.

    Conclusions:

    • Vitamin C is critical for maintaining normal spermatogenesis.
    • Vitamin C deficiency may contribute to male infertility through increased spermatocyte apoptosis.
    • Heat-shock protein 70's absence is a potential biomarker for vitamin C-related male reproductive dysfunction.