Related Experiment Video
Updated: Aug 7, 2026

Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Somatic cell and sperm cell cytogenetics in a patient with t(14; 21)
Yan-Ping Tang1, Xiu-Sheng Liu, Yuan Liu
1Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Abstract:
Approximately 15%-20% of clinically recognizable pregnancies end in spontaneous abortion. About half of the spontaneous abortions in the early stage of the pregnancy are due to chromosomal abnormalities. Using GTG chromosome banding and dual-color fluorescence in situ hybridization (FISH) techniques, we determined the cytogenetic aberration in the husband of a couple with spontaneous recurrent abortions. Karyotype analysis showed 46, XX in the wife and 45, XY, -14, -21, +t(14; 21) in the husband. We studied the mechanism of formation of the abnormal chromosome with Robertsonian translocation between chromosomes 14 and 21 by FISH and flow cytometric sorting in the sperm cells. The result showed that 71% of the gametes were balanced and the remaining 29% were not. As a result, the couple was given genetic counseling.
Related Concept Videos
Karyotyping
Meiosis I
Methods of Nuclear Reprogramming
Nondisjunction
Nondisjunction
Cancers Originate from Somatic Mutations in a Single Cell

