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Updated: Aug 6, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Identification of histidine-60 interaction with copper in activation of Chironomidae ferrochelatase
1Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong.
Abstract:
Site-directed mutagenesis study of the conserved residue in ferrochelatase of chironomidae showed the binding interaction of copper with histidine-60. The activities of the variants increase by > 4-fold with H60N and 2 fold with H60D. The study identifies for the first time that the highly conserved H60 is a key molecular determinant in directing a catalytically competent mode of metal binding in the active site.
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