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Blood-CSF barrier function in the rat embryo.

P A Johansson1, K M Dziegielewska, C J Ek

  • 1Department of Pharmacology & Centre for Neuroscience, University of Melbourne, Parkville, Victoria 3010, Australia.

The European Journal of Neuroscience
|June 28, 2006
PubMed
Summary
This summary is machine-generated.

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The embryonic blood-cerebrospinal fluid barrier matures early, with ventricular expansion influencing marker concentrations more than permeability changes. Selective transfer mechanisms exist for proteins and small molecules.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Physiology

Background:

  • The blood-cerebrospinal fluid (CSF) barrier is crucial for brain homeostasis.
  • Understanding its development in embryonic mammals is essential for developmental neuroscience.

Purpose of the Study:

  • To investigate the maturation of the blood-CSF barrier function and ventricular system expansion in embryonic rats.
  • To determine the mechanisms of solute and protein transfer across the developing barrier.

Main Methods:

  • Injected permeability markers (sucrose, inulin) and measured concentrations in plasma and CSF of embryonic rats (E12-18).
  • Assessed total protein concentrations and compared transfer of human vs. bovine albumin.
  • Utilized ultrastructural analysis and immunocytochemistry to examine barrier integrity and cellular transfer.

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Main Results:

  • Blood-CSF barrier tight junctions are impermeable to small molecules by E15, indicating early maturation.
  • Decreasing CSF/plasma ratios for small molecules correlate with ventricular volume expansion, not permeability changes.
  • Selective, transcellular transfer mechanisms for proteins and small molecules were identified.

Conclusions:

  • The embryonic blood-CSF barrier is morphologically and functionally mature early in development.
  • Ventricular expansion significantly impacts the distribution of passively diffusing molecules.
  • Distinct transcellular pathways facilitate the transfer of proteins and small molecules across the embryonic blood-CSF interface.