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Related Experiment Videos

Somatostatin release from rat cerebral cortex synaptosomes.

G Bonanno1, B Parodi, S Cafaggi

  • 1Istituto di Farmacologia e Farmacognosia, Università degli Studi di Genova, Italy.

Journal of Neurochemistry
|October 1, 1991
PubMed
Summary

This study shows that depolarizing rat cerebral cortex synaptosomes releases somatostatin-like immunoreactivity (SRIF-LI), primarily somatostatin-14. This release is dependent on calcium ions and sensitive to tetrodotoxin and sodium levels.

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Area of Science:

  • Neuroscience
  • Neurochemistry

Background:

  • Somatostatin-like immunoreactivity (SRIF-LI) is present in rat cerebral cortex synaptosomes.
  • Understanding the release mechanisms of SRIF-LI is crucial for comprehending its role in neuronal function.

Purpose of the Study:

  • To investigate the release of SRIF-LI from rat cerebral cortex synaptosomes in response to various depolarizing stimuli.
  • To elucidate the ionic and pharmacological requirements for SRIF-LI release.

Main Methods:

  • Superfusion of rat cerebral cortex synaptosomes.
  • Stimulation with high KCl, veratrine, and sodium-deprived media.
  • Measurement of SRIF-LI release using radioimmunoassay.
  • High-performance liquid chromatography (HPLC) analysis of released SRIF forms.

Related Experiment Videos

  • Assessment of calcium, sodium, and tetrodotoxin dependency.

    • Main Results:

    • High KCl and veratrine evoked concentration-dependent SRIF-LI release, which was abolished by calcium omission.
    • Calcium ions were essential for K+-evoked SRIF-LI release, with release increasing up to 1.2 mM Ca2+.
    • Tetrodotoxin partially inhibited K+-evoked release, suggesting involvement of voltage-sensitive sodium channels.
    • Sodium-deprived media accelerated spontaneous SRIF-LI outflow, independent of calcium.
    • HPLC analysis revealed that somatostatin-14 constituted over 90% of the released SRIF-LI.

    Conclusions:

    • SRIF-LI release from rat cerebral cortex synaptosomes is a calcium-dependent process.
    • Voltage-sensitive sodium channels play a role in stimulus-evoked SRIF-LI release.
    • Somatostatin-14 is the predominant form of somatostatin released under these experimental conditions.