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An eco-friendly and sustainable process for enzymatic hydrolysis of penicillin G in cloud point system
Li Wang1, Zhilong Wang, Jian-He Xu
1School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Abstract:
Enzymatic hydrolysis of penicillin G by immobilized penicillin acylase in a nonionic surfactant mediated cloud point system was presented. The effect of the operation parameters on equilibrium pH of this enzymatic hydrolysis process without pH control was examined. A relatively high equilibrium pH in cloud point system without pH control can be obtained. The feasibility of recycling utilization of the nonionic surfactant, a novel green solvent, was also investigated experimentally. Enzymatic hydrolysis of penicillin G in a discrete semi-batch mode, which simulates a semi-continuous process, envisages a completely eco-friendly, sustainable and efficient process for production of 6-aminopenicillanic acid.
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