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Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
Published on: September 27, 2024
Effects of the eye phase in DNA unzipping
Debaprasad Giri1, Sanjay Kumar
1Physics Section, MMV, Banaras Hindu University, Varanasi 221 005, India.
Abstract:
The onset of the "eye phase" (a phase consisting of configurations of eye-type conformations or bubbles in the double-stranded DNA) and its role during the DNA unzipping is studied when a force is applied to the interior of the chain. The directionality of the hydrogen bond introduced here shows oscillations in force-extension curve similar to a "sawtooth" kind of oscillations seen in the protein unfolding experiments. The effects of intermediates (hairpins) and stacking energies on the melting profile have also been discussed.
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