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Related Experiment Videos

Non-side-population hematopoietic stem cells in mouse bone marrow.

Yohei Morita1, Hideo Ema, Satoshi Yamazaki

  • 1Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639 Japan.

Blood
|June 29, 2006
PubMed
Summary

Hematopoietic stem cells (HSCs) exist in both side population (SP) and main population (MP) fractions. Both SP and MP HSCs demonstrate similar repopulating capacity and self-renewal, challenging the SP

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Hematopoietic stem cells (HSCs) are crucial for blood cell formation.
  • HSCs are traditionally thought to reside primarily in the side population (SP) fraction of adult mouse bone marrow (BM).
  • The SP phenotype is often associated with multidrug resistance and stem cell function.

Purpose of the Study:

  • To investigate the presence and function of non-side population (non-SP) HSCs.
  • To compare the characteristics and repopulating capacities of SP and non-SP HSCs.
  • To determine if the SP phenotype is essential for HSC function.

Main Methods:

  • Hoechst 33342 dye staining to identify SP and main population (MP) cells.
  • Isolation of CD34(-)c-Kit(+)Sca-1(+)lineage marker(-) (CD34(-)KSL) cells.

Related Experiment Videos

  • Competitive repopulation assays and secondary transplantation.
  • Cell-cycle analysis and Bcrp-1 expression analysis.

    • Main Results:

    • The CD34(-)KSL cell population, enriched for HSCs, was equally divided into SP and MP fractions.
    • SP and MP CD34(-)KSL cells exhibited similar repopulating activity and self-renewal capacity.
    • SP and MP phenotypes were found to be interconvertible.
    • Both SP and MP HSCs displayed quiescent cell-cycle states and uniform kinetics.
    • Similar Bcrp-1 expression was observed in both SP and MP HSCs.

    Conclusions:

    • The side population (SP) phenotype does not exclusively define all hematopoietic stem cells (HSCs).
    • Non-SP HSCs possess comparable stem cell functions to SP HSCs.
    • HSC function is not solely dependent on the SP phenotype, suggesting other regulatory factors are involved.