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Related Experiment Videos

Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor.

Zhiyu Zhang1, Paolo Boccazzi, Hyun-Goo Choi

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave. 66-566, Cambridge, MA 02139, USA.

Lab on a Chip
|June 29, 2006
PubMed
Summary

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This study presents a novel polymer-based microbioreactor for continuous microbial cell cultivation. It effectively minimizes cell adhesion and wall growth, enabling stable, long-term steady-state conditions for research.

Area of Science:

  • Biotechnology
  • Microbial Physiology
  • Bioengineering

Background:

  • Continuous culture in chemostats is vital for studying microbial steady states.
  • Microbioreactors offer advantages for high-throughput biological research.
  • Cell adhesion and wall growth are challenges in microscale continuous cultivation.

Purpose of the Study:

  • To develop a polymer-based microbioreactor system for continuous microbial cultivation.
  • To integrate real-time monitoring of optical density (OD), pH, and dissolved oxygen (DO).
  • To mitigate non-specific cell adhesion and wall growth in microscale reactors.

Main Methods:

  • Utilized a 150 microL membrane-aerated, well-mixed microbioreactor.
  • Employed pressure-driven flow for medium supply and local heating to prevent back growth.

Related Experiment Videos

  • Modified microbioreactor surfaces with poly(ethylene glycol)-grafted poly(acrylic acid) copolymer films.

    • Main Results:

    • Achieved stable, continuous cultivation of Escherichia coli (E. coli).
    • Demonstrated significantly reduced wall growth on modified bio-inert surfaces.
    • Successfully maintained steady-state conditions across various dilution rates, confirmed by stable OD, pH, and DO.

    Conclusions:

    • The developed polymer-based microbioreactor is effective for long-term, stable continuous cultivation of microbial cells.
    • Bio-inert surface modification is crucial for preventing cell adhesion and ensuring reliable microscale reactor performance.
    • The system provides a robust platform for microbial physiology research under controlled, steady-state conditions.