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Related Experiment Videos

Bladder tissue formation from cultured bladder urothelium.

Siam Oottamasathien1, Karin Williams, Omar E Franco

  • 1Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, USA. siam.oottamasathien@vanderbilt.edu

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|June 29, 2006
PubMed
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Researchers successfully created chimeric bladder tissue using cultured urothelium and embryonic mesenchyme. This novel tissue recombination model offers a new tool for studying bladder development and disease mechanisms.

Area of Science:

  • Urology
  • Developmental Biology
  • Tissue Engineering

Background:

  • Tissue recombination is vital for studying organ development via paracrine signaling.
  • Previous studies cultured rodent bladder urothelium but did not utilize it in recombination models.
  • A method to recapitulate bladder tissue using primary cultured urothelium was lacking.

Purpose of the Study:

  • To determine if primary cultured bladder urothelium can form bladder tissue when recombined with embryonic bladder mesenchyme.
  • To establish a novel tissue recombination model for bladder research.

Main Methods:

  • Adult rat bladder urothelium was isolated, cultured, and expanded.
  • Expanded urothelium was recombined with embryonic day-14 mouse bladder mesenchyme.

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  • Recombinant tissues were grafted under the renal capsule of immunocompromised mice and harvested after 28 days.
  • Tissues were analyzed using histology and immunohistochemistry.

    • Main Results:

    • Immunocytochemistry confirmed pure urothelial cultures, free of mesenchymal contamination.
    • Histological and immunohistochemical analysis of grafts showed mature urothelium and stromal differentiation, indicating successful bladder tissue formation.
    • Control grafts (mesenchyme alone, urothelium alone, or matrix alone) did not form bladder tissue.

    Conclusions:

    • Primary cultured bladder urothelium, when recombined with embryonic bladder mesenchyme, can successfully form chimeric bladder tissue in vivo.
    • This study presents a powerful new tool for investigating bladder development and disease.
    • Future research can utilize this model for in vitro genetic manipulation of urothelium to study its effects on bladder growth and development.