Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MLEM deconvolution of protein X-ray diffraction images based on a multiple-PSF model.

Daan Zhu1, Moe Razaz, Andrew Hemmings

  • 1School of Computing Sciences, University of East Anglia, Norwich NR4 7TJ, UK. d.zhu@uea.ac.uk

IEEE Transactions on Nanobioscience
|June 30, 2006
PubMed
Summary

This study introduces a new multiple point spread function (PSF) model to correct diffuse light distortion (DLD) in protein X-ray diffraction (XRD) images. The improved method enhances image quality, reducing errors in spot integration and electron density maps.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system.

Acta crystallographica. Section D, Structural biology·2025
Same author

Influence of lighting on sleep behaviour, circadian rhythm and spontaneous blink rate in stabled riding school horses (Equus caballus).

PloS one·2025
Same author

Treatment of industrial ferric sludge through a facile acid-assisted hydrothermal reaction: Focusing on dry mass reduction and hydrochar recyclability performance.

The Science of the total environment·2023
Same author

Transformation of polyvinyl chloride (PVC) into a versatile and efficient adsorbent of Cu(II) cations and Cr(VI) anions through hydrothermal treatment and sulfonation.

Journal of hazardous materials·2021
Same author

Stabilizing lactate production through repeated batch fermentation of food waste and waste activated sludge.

Bioresource technology·2020
Same author

Causal and functional interpretation of mu- and delta-opioid receptor profiles in mesoaccumbens and nigrostriatal pathways of an oral stereotypy phenotype.

Behavioural brain research·2018

Area of Science:

  • Structural biology
  • Biophysics
  • Crystallography

Background:

  • Protein X-ray diffraction (XRD) images can be degraded by diffuse light distortion (DLD).
  • Accurate image data is crucial for determining protein structures.
  • Existing methods may not fully correct complex distortions.

Purpose of the Study:

  • To develop and validate a novel method for correcting DLD in protein XRD data.
  • To improve the quality of restored XRD images and subsequent structural analysis.
  • To introduce a multiple point spread function (PSF) model for enhanced deconvolution.

Main Methods:

  • Collected raw PSFs from isolated spots on diffraction patterns to characterize DLD orientation.
  • Applied adaptive ridge regression (ARR) to denoise raw PSF data.

Related Experiment Videos

  • Modeled raw PSFs using a target Gaussian function.
  • Employed a maximum likelihood expectation maximization (MLEM) algorithm with a multi-PSF model for image restoration.

    • Main Results:

    • The multiple PSF model significantly improved the quality of restored XRD data compared to a single PSF model.
    • Restoration using the multi-PSF model led to reduced spot integration error (chi-squared).
    • Improved XRD data resulted in better electron density maps.

    Conclusions:

    • A multiple PSF model effectively corrects diffuse light distortion in protein XRD images.
    • This approach enhances the accuracy of structural determination from diffraction data.
    • The developed method offers a significant advancement in processing challenging XRD datasets.