Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surviving the breakup: the DNA damage checkpoint.

Jacob C Harrison1, James E Haber

  • 1Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02445, USA. harrison@brandeis.edu

Annual Review of Genetics
|June 30, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutations and structural variants arising during double-strand break repair.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Learning about break-induced replication from bacteriophages.

Nature reviews. Molecular cell biology·2025
Same author

Arp2/3 and type-I myosins control chromosome mobility and end-resection at double-strand breaks in S. cerevisiae.

Nature communications·2025
Same author

The Mediator Kinase Module regulates cell cycle re-entry and transcriptional responses following DNA damage.

bioRxiv : the preprint server for biology·2025
Same author

Mutations and structural variants arising during double-strand break repair.

bioRxiv : the preprint server for biology·2025
Same author

Prolonged cell cycle arrest in response to DNA damage in yeast requires the maintenance of DNA damage signaling and the spindle assembly checkpoint.

eLife·2024

Cells activate the DNA damage checkpoint to arrest the cell cycle, allowing DNA repair and maintaining genomic stability. This process involves kinases like ATM and ATR, and chromatin modifications, with distinct recovery and adaptation mechanisms.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cells possess a DNA damage checkpoint to respond to chromosomal double-strand breaks.
  • This checkpoint induces cell cycle arrest, facilitating DNA repair and preventing genomic instability.
  • Key players include ATM and ATR kinases, crucial for checkpoint activation and effector signaling.

Purpose of the Study:

  • To elucidate the molecular mechanisms of DNA damage checkpoint activation.
  • To understand the role of chromatin modification in checkpoint processes.
  • To differentiate between checkpoint recovery and adaptation mechanisms.

Main Methods:

  • Focus on molecular mechanisms of checkpoint activation.
  • Investigating recruitment of ATM and ATR kinases to damaged DNA.

Related Experiment Videos

  • Analyzing chromatin modifications during checkpoint response.

    • Main Results:

    • Checkpoint activation involves recruitment of ATM and ATR kinases to damaged DNA sites.
    • Chromatin modification is integral to checkpoint activation and maintenance.
    • Distinct processes of recovery (inactivation post-repair) and adaptation (re-entry with unrepairable damage) exist.

    Conclusions:

    • The DNA damage checkpoint is essential for genomic stability.
    • Understanding kinase recruitment and chromatin modification provides insight into checkpoint control.
    • Cellular responses to DNA damage include regulated inactivation and controlled re-entry.