Related Experiment Video
Updated: Aug 7, 2026

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
Published on: December 11, 2020
Hydrogen peroxide inhibits activity of the IGF-1 receptor kinase
Thomas L Schmitt1, Helmut Klein, Wulf Dröge
1Division of Immunochemistry, Deutsches Krebsforschungszentrum, Heidelberg, Germany. T.Schmitt@dkfz.de
Abstract:
IGF-1 receptor (IGF1R) is a transmembrane tyrosine kinase, which is indispensable for cellular growth and differentiation. Using a recombinant GST-tagged cytosolic fragment of IGF1R (GST-IGFK), we now show that oxidation by low doses (50 muM) of hydrogen peroxide markedly inhibits maximum phosphate incorporation in autophosphorylation and substrate phosphorylation assays. A similar inhibition was observed on the activity of intact IGF1R after treatment of T-47D cells. These results are in sharp contrast to the positive influence of hydrogen peroxide on the highly homologous insulin receptor kinase, which was assayed for comparison. This reciprocal influence of physiologically relevant doses of hydrogen peroxide may have important implications on signal transduction of the closely related receptors for insulin and IGF-1.
Related Concept Videos
Regulation of Angiogenesis and Blood Supply
Peroxisomes
The JAK-STAT Signaling Pathway
Amplifying Signals via Enzymatic Cascade
TGF - β Signaling Pathway
Insulin: The Receptor and Signaling Pathways