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Updated: Aug 7, 2026
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Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
Published on: June 22, 2016
[Isolation and function analysis of rat CD4+ CD25+ regulatory T cells]
Ling Lu1, Feng Zhang, Xue-hao Wang
1Center of Liver Transplantation, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. lvling_vip@yahoo.com.cn
Summary
Researchers developed an effective method to isolate rat CD4(+) CD25(+) regulatory T cells using magnetic bead cell sorting. These cells effectively suppress T cell proliferation and express the Foxp3 gene.
Area of Science:
- Immunology
- Cell Biology
Context:
- Regulatory T cells (Tregs) play a crucial role in maintaining immune homeostasis.
- Efficient isolation of functional Tregs is essential for studying their immunomodulatory properties.
Purpose:
- To establish and validate a method for isolating rat CD4(+) CD25(+) regulatory T cells.
- To analyze the purity, gene expression, and suppressive function of isolated Tregs.
Summary:
- Rat lymphocytes were isolated from spleens, and CD4(+) CD25(+) T cells were purified using magnetic bead cell sorting (MACS).
- Purity was confirmed by flow cytometry (FCM), Foxp3 gene expression by RT-PCR, and suppressive function via mixed lymphocyte reaction (MLR).
- Isolated Tregs demonstrated high purity (86-93%), expressed Foxp3, suppressed CD4(+) CD25(-) T cell proliferation and IFN-gamma/IL-2 production, while secreting IL-10.
Impact:
- This study provides an effective protocol for obtaining highly pure and functional rat CD4(+) CD25(+) regulatory T cells.
- The validated method facilitates further research into Treg-mediated immune suppression and potential therapeutic applications.
