Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic changes in CA1 dendritic spines associated with ischemic tolerance.

Dale Corbett1, Tina Giles, Suzanne Evans

  • 1Division of Basic Medical Sciences, Faculty of Medicine, Memorial University, St. John's, NL Canada, A1B 3V6. corbett@mun.ca

Experimental Neurology
|June 30, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GPNMB-directed CAR T cell therapy against MiT/TFE-family fusion-driven solid tumors.

Nature cancer·2026
Same author

In Memorium: Dr. Michelle Ploughman.

Neurorehabilitation and neural repair·2026
Same author

Cilia beating of ependymal cells regulates adult neural stem cell quiescence via mechanical forces mediated by PKD1/2-TRPM3.

Neuron·2026
Same author

Immature Neutrophil Programs Associate with Burn Mortality and Extend Across Critical Illnesses.

Journal of burn care & research : official publication of the American Burn Association·2026
Same author

Comparing the clinical and cost-effectiveness of various washout policies in preventing catheter associated complications in adults living with long-term catheters: synopsis of the CATHETER II RCT.

Health technology assessment (Winchester, England)·2026
Same author

Plagl1 regulates the retinal progenitor cell to Müller glial cell transition.

PLoS genetics·2026

Ischemic preconditioning protects brain cells from stroke damage. This study shows that changes in dendritic spines are key to recovery of function after ischemic tolerance.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Stroke Research

Background:

  • Hippocampal CA1 neurons are highly susceptible to global ischemia.
  • Ischemic tolerance can be induced by brief prior ischemic episodes (ischemic preconditioning).
  • Dendritic spines are crucial for neuroplasticity, memory, and functional recovery.

Purpose of the Study:

  • To investigate changes in hippocampal CA1 dendritic spines during ischemic tolerance development and post-ischemic recovery.
  • To correlate dendritic spine alterations with functional recovery after ischemic preconditioning.

Main Methods:

  • Gerbils underwent ischemic preconditioning (two 1.5 min occlusions) followed by a 5 min global ischemia.
  • Spine densities in CA1 apical and basilar dendrites were quantified using Golgi-Cox staining at various survival times (3, 10, 30 days).

Related Experiment Videos

  • Open-field habituation was assessed to evaluate CA1 functional integrity.

    • Main Results:

    • Animals with ischemic tolerance (IP10, IP30) and post-occlusion (PO) groups showed significantly higher CA1 dendritic spine densities compared to controls.
    • Initially, tolerant animals exhibited behavioral impairments (habituation deficits) coinciding with reduced spine densities.
    • Behavioral deficits resolved over time as dendritic spine densities increased.

    Conclusions:

    • Dendritic spine density changes are associated with the development of ischemic tolerance.
    • Increased dendritic spine density correlates with functional recovery after ischemic events.
    • Dendritic spines likely play a significant role in the recovery of function observed in ischemic tolerance and stroke.