High-resolution analysis of catechol-type siderophores using polyamide thin layer chromatography
Xiaojun Xie1, Jingguo Wang, Hongli Yuan
1Department of Plant Nutrition, College of Natural Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.
Abstract:
The iron-deficient culture supernatant of a soil bacterial strain identified as Erwinia sp. was analyzed using a new high-resolution polyamide thin layer chromatography (TLC) and a silica TLC. The results showed both TLC methods were very effective for separating simple catechol compounds such as 2,3-dihydroxybenzoic acid (2,3-DHBA) and catechol. However, in the analysis of more complicated catechol compounds or true catechol-type siderophores (conjugates of 2,3-DHBA and amino acids), the polyamide TLC had the higher resolution. Polyamide TLC analysis showed that strain S1 produced three distinct catechol-type siderophores.
