Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorogenic ester substrates to assess proteolytic activity.

Laurent Mugherli1, Olga N Burchak, François Chatelain

  • 1Laboratoire Biopuces, Département Réponse et Dynamique Cellulaires, Commissariat à l'Energie Atomique, Grenoble, France.

Bioorganic & Medicinal Chemistry Letters
|June 30, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Digital light processing programs shape-morphing hydrogels into undulating 3D scaffolds supporting corneal limbal epithelial organization.

Acta biomaterialia·2026
Same author

Self-Organization of Long-Lasting Human Endothelial Capillary-Like Networks Guided by DLP Bioprinting.

Advanced healthcare materials·2024
Same author

In vitro proliferation and long-term preservation of functional primary rat hepatocytes in cell fibers.

Scientific reports·2022
Same author

Correction: Mechanical stress shapes the cancer cell response to neddylation inhibition.

Journal of experimental & clinical cancer research : CR·2022
Same author

Mechanical stress shapes the cancer cell response to neddylation inhibition.

Journal of experimental & clinical cancer research : CR·2022
Same author

Construction of functional biliary epithelial branched networks with predefined geometry using digital light stereolithography.

Biomaterials·2021
Same journal

Discovery of a potent sGC stimulator with once-daily dosing potential for the treatment of hypertension.

Bioorganic & medicinal chemistry letters·2026
Same journal

OracleScreen-LILRB4 (HTS-Oracle v3): machine learning-guided discovery of myeloid immune checkpoint binders validated in patient-derived cells.

Bioorganic & medicinal chemistry letters·2026
Same journal

Rational design and N-terminal acylation of a lactam-cyclized 11-residue antimicrobial peptide for improved antibacterial potency.

Bioorganic & medicinal chemistry letters·2026
Same journal

Rational design, synthesis, and biological evaluation of chalcone hybrids including benzoylpiperazin (phenylacetylpiperazin)-thiophene as anti- Alzheimer's agents.

Bioorganic & medicinal chemistry letters·2026
Same journal

New aminopiperidine-azoles as antifungal agents against Candida albicans with mechanistic insights into the cAMP-PKA pathway.

Bioorganic & medicinal chemistry letters·2026
Same journal

Novel 1H-pyrrole-2,5-dione derivatives as potential anti-diabetic agents: design, synthesis, in vitro, in ovo, and molecular docking studies.

Bioorganic & medicinal chemistry letters·2026
See all related articles

Researchers developed new fluorogenic ester substrates from fluorescein. These substrates detect protease activity and can be used for screening protease inhibitors.

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Enzymology

Background:

  • Proteases are crucial enzymes involved in various biological processes.
  • Developing sensitive and versatile tools for protease activity detection is essential.
  • Existing substrates may lack broad applicability or sensitivity.

Purpose of the Study:

  • To synthesize novel fluorogenic ester substrates for protease detection.
  • To demonstrate the broad utility of these substrates across different protease families.
  • To establish the substrates as a tool for protease inhibitor screening.

Main Methods:

  • Multi-step synthesis of fluorogenic ester substrates using fluorescein and common precursors.
  • Characterization of substrate properties, including fluorescence generation upon enzymatic cleavage.

Related Experiment Videos

  • Testing substrate performance with various proteases from serine and cysteine superfamilies.
  • Evaluation of substrate sensitivity to specific protease inhibitors.

    • Main Results:

    • Successful synthesis of new fluorogenic ester substrates.
    • Generation of bright green fluorescence upon proteolysis by various proteases.
    • Demonstrated broad applicability of a single substrate for different protease superfamilies.
    • Confirmed sensitivity of substrate cleavage to specific protease inhibitors.

    Conclusions:

    • The novel fluorogenic ester substrates are effective tools for detecting protease activity.
    • These substrates offer a versatile platform for studying diverse proteases.
    • The developed substrates are valuable for high-throughput screening of protease inhibitors.