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Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis
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Organ specific apoptosis following polymicrobial intraperitoneal infection.

G A Franklin1, M Turina, J F Kuhn

  • 1Veterans Affairs Medical Center, University of Louisville, Ambulatory Care Building, 550 South Jackson Street, Louisville, KY 40292, USA.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|June 30, 2006
PubMed
Summary

Intra-abdominal infection causes organ-specific changes in leukocyte apoptosis. Apoptotic cell numbers increased significantly in the liver and spleen, aiding inflammation resolution.

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Area of Science:

  • Immunology
  • Cell Biology
  • Pathology

Background:

  • Leukocyte apoptosis is crucial for removing harmful cells and resolving inflammation.
  • Intra-abdominal infection can disrupt normal apoptotic processes.

Purpose of the Study:

  • To investigate the organ-specific distribution of apoptotic cells in response to intra-abdominal infection.
  • To determine if apoptotic cell numbers change disproportionately in different parenchymal organs.

Main Methods:

  • Cecal ligation and puncture (CLP) model in mice to induce intra-abdominal infection.
  • Quantification of apoptotic cells in the liver, spleen, lung, and peripheral blood.
  • Measurement of myeloperoxidase (MPO) levels as an indicator of neutrophil infiltration.

Main Results:

  • Liver and spleen MPO levels consistently increased post-CLP.
  • Lung MPO levels remained low throughout the study.
  • Apoptotic cell percentages in the liver and spleen increased significantly over 9 days, while lung apoptosis remained unchanged.

Conclusions:

  • Apoptotic cell distribution during intra-abdominal infection is organ-specific.
  • Increased apoptosis in the liver and spleen suggests a role in clearing inflammatory cells.
  • These findings highlight the spleen and liver's importance in resolving localized infection.