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Published on: January 27, 2014
A hyaluronidase supercatalyst for the enzymatic polymerization to synthesize glycosaminoglycans
Shiro Kobayashi1, Masashi Ohmae, Hirofumi Ochiai
1Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan. kobayash@kit.ac.jp
Abstract:
Hyaluronidase (HAase) catalyzes multiple enzymatic polymerizations with controlling regio- and stereoselectivity perfectly. This behavior, that is, the single enzyme being effective for multireactions and retaining the enzyme catalytic specificity, is not usual, and hence, HAase is a supercatalyst. Various sugar oxazoline monomers prepared based on the concept "transition-state analogue substrate" were successfully polymerized and copolymerized with HAase catalysis, yielding natural and unnatural glycosaminoglycans.
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