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Related Experiment Videos

Lactate stimulates endothelial cell migration.

Stefan Beckert1, Farshid Farrahi, Rummana S Aslam

  • 1Department of Surgery, University of California, San Francisco, California, USA, and Department of General Surgery, University Hospital Tübingen, Germany. stefan.beckert@med.uni-tuebingen.de

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society
|July 1, 2006
PubMed
Summary
This summary is machine-generated.

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High lactate levels in healing wounds promote endothelial cell migration by enhancing vascular endothelial growth factor (VEGF) production. This study clarifies lactate

Area of Science:

  • Biochemistry
  • Cell Biology
  • Wound Healing Research

Background:

  • High lactate levels are characteristic of healing wounds, but their precise role remains unclear.
  • Lactate is known to stimulate collagen synthesis and vascular endothelial growth factor (VEGF) production.
  • VEGF is crucial for endothelial cell migration, a key process in wound repair.

Purpose of the Study:

  • To investigate the correlation between accumulated lactate and the biological activity of VEGF.
  • To determine the effect of lactate on endothelial cell migration.

Main Methods:

  • Human umbilical vein and microvascular endothelial cells were cultured and serum-starved.
  • Cells were treated with varying concentrations of l-lactate.
  • Cell migration was assessed using a modified Boyden chamber assay, and VEGF protein levels were measured via enzyme-linked immunoassay.

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Main Results:

  • Lactate enhanced VEGF protein synthesis in a time- and dose-dependent manner.
  • Lactate alone did not induce cell migration, but significantly increased it when co-administered with endothelial cells in the Boyden chamber.
  • The migratory effect of lactate was inhibited by anti-VEGF antibodies and cycloheximide.

Conclusions:

  • Lactate enhances VEGF production in endothelial cells.
  • Lactate itself is not a chemoattractant for endothelial cells.
  • Lactate-mediated endothelial cell migration is dependent on VEGF regulation.