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Related Experiment Videos

Bicarbonate kinetics in Indian males.

T Raj1, R Kuriyan, A V Kurpad

  • 1Division of Nutrition, St John's Institute of Population Health and Clinical Research, St John's National Academy of Health Sciences, Bangalore 560 034, India.

Journal of Biosciences
|July 1, 2006
PubMed
Summary

Measuring substrate oxidation rates requires accurate tracer recovery. This study reveals that bicarbonate recovery differs between fasted and fed states, impacting oxidation rate calculations and highlighting significant metabolic shifts.

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Area of Science:

  • Metabolic studies
  • Tracer kinetics
  • Biochemistry

Background:

  • In vivo substrate oxidation measurements rely on tracer data, often using expired CO2.
  • Incomplete recovery of 13C-labelled CO2 can lead to underestimation of substrate oxidation rates.
  • Correction for incomplete tracer recovery is necessary, typically via bicarbonate infusion.

Purpose of the Study:

  • To study tracer-labelled bicarbonate recovery using a bolus administration model.
  • To characterize bicarbonate kinetics using a three-compartment model.
  • To assess changes in compartmental fluxes between fasted and fed states.

Main Methods:

  • Bolus administration of tracer-labelled bicarbonate.
  • Three-compartment modeling of bicarbonate kinetics.

Related Experiment Videos

  • Comparison of bicarbonate recovery in fasted versus fed states.

    • Main Results:

    • Bicarbonate recovery was lower in both fasted (69%) and fed (67%) states compared to continuous infusions (71-74%).
    • A 20-fold increase in bicarbonate flux was observed between the central and visceral compartments during feeding.
    • Fasted and fed states showed distinct bicarbonate recovery patterns and significant flux changes.

    Conclusions:

    • Tracer bicarbonate recovery differences between fasted and fed states are comparable to continuous infusion findings.
    • Bicarbonate fluxes undergo substantial changes across body compartments based on metabolic state.
    • Accurate assessment of substrate oxidation requires accounting for metabolic state-dependent bicarbonate kinetics.