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Modified His-tag fusion vector for enhanced protein purification by immobilized metal affinity chromatography

Sinéad T Loughran1, Noeleen B Loughran, Barry J Ryan

  • 1School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland.

Analytical Biochemistry
|July 4, 2006
PubMed
Abstract

No abstract available in PubMed .

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...