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2,6-diisopropylphenol, a general anesthetic, inhibits glutamate action on rat synaptosomes
M Bianchi1, T Battistin, L Galzigna
1Department of Biological Chemistry, University of Padova, Italy.
Neurochemical Research
|April 1, 1991
Abstract:
2,6-diisopropylphenol (propofol), a general intravenous anesthetic, inhibits the glutamate-dependent Ca2+ entry in rat synaptosomes with an approximate IC50 of 3.0 x 10(-5) M. Propofol, at concentrations above 10(-6) M, also inhibits the ATP-dependent uptake of glutamate in the presence of Ca2+, with an approximate IC50 of 3.5 x 10(-5) M, while it only has a slight inhibitory effect on the release of glutamate. The ouabain-insensitive synaptosomal ATPase is strongly inhibited by propofol, with an IC50 of about 2.5 x 10(-6) M, at concentrations which do not affect the luciferase system.