Iontophoretic study of medial septal neurons in the unanesthetized rat

The effects of the iontophoretic applications of glutamate (Glu), gamma-aminobutyric acid (GABA), acetylcholine (ACh) and carbachol (CARB) were studied on neurons located in the medium septal area (MSA) in the unanesthetized rat. In the absence of anesthesia, functional properties of the MSA neurons were significantly different from those observed in the urethane-anesthetized rat (higher variability of discharge rate, lower percentage of rhythmically bursting neurons). Glu excited 80% and GABA inhibited 96% of the MSA neurons. These percentages were similar to those obtained in the urethane-anesthetized rats. In contrast, the percentage of neurons excited by ACh (28%) or by CARB (27.2%) were significantly lower than in the urethane-anesthetized rat. Our results suggest that urethane might alter cholinergic sensitivity in the MSA and confirm that anesthesia can induce a bias in the iontophoretic study of some brain structures.