Altered neuroantigen-specific cytokine secretion in a Th2 environment reduces experimental autoimmune
Stefanie J Kirwin1, Kenichi C Dowdell, Claudia Hindinger
1Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 1333 San Pablo Ave, Los Angeles, CA 90033, USA.
Abstract:
Activation of Th2 cells suppresses clinical experimental autoimmune encephalitis (EAE), demyelination and expression of genes associated with Th1-mediated inflammation. Despite both reduced central nervous system inflammation and IFN-gamma induced MHC class II expression by microglia, the composition of CNS infiltrates in Th2-protected mice were similar to mice with EAE. Analysis of the CNS infiltrating cells by flow cytometry suggests that protection did not correlate with abrogation of CD4+ T cell recruitment, preferential recruitment of donor Th2 cells or an increased frequency of CD25+ CD4+ T cells. By contrast, protection correlated with an increased frequency of neuroantigen-specific Th2 cells infiltrating the CNS. These data suggest that a peripheral Th2 cytokine environment influences both potential antigen presenting cells as well as recruitment and/or retention of neuroAg-specific Th2 CD4+ T cells.
