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Updated: Aug 7, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
Pro-antibiotic substrates for the identification of enantioselective hydrolases
Bum-Yeol Hwang1, Jong-Min Oh, Juhan Kim
1School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Korea.
Abstract:
A new growth-based screening method for the identification of enantioselective hydrolases, such as lipases and esterases, using pro-antibiotic substrates was devised. An enantioselective hydrolase could be identified by measuring growth rates of cells in liquid media containing (R)- or (S)-2-phenylbutyric chloramphenicol esters. This method can be applied to the screening of novel enantioselective microbes and to the high-throughput screening for the directed evolution of enantioselective hydrolytic enzymes.
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