Related Experiment Video
Updated: Aug 7, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Amino-terminal processing of the human cannabinoid receptor 1
Rickard Nordström1, Helena Andersson
1Department of Biosciences and Nutrition at Novum, Karolinska Institute, Huddinge, Sweden.
Abstract:
The human cannabinoid receptor 1 (CB1), a G protein-coupled receptor (GPCR), translocates its long amino-terminal (N-terminal) domain across the endoplasmic reticulum (ER) membrane in a C-to-N terminal direction. Using the Semliki Forest virus (SFV) expression system, CB1 was expressed in baby hamster kidney (BHK) cells. It was found that a large fraction of the CB1 molecules were N-terminally truncated prior to ER translocation. Truncation was fast and independent of the proteasome. It is concluded that the truncation process might be a way to create a novel type of CB1.
Related Concept Videos
Receptor-mediated Endocytosis
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Receptor-mediated Endocytosis
tRNA Activation
G-protein Coupled Receptors
G-protein Coupled Receptors
Mitochondrial Precursor Proteins
Most of the mitochondrial precursors...

