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Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
Published on: September 28, 2022
In vivo transduction of an Stx-encoding phage in ruminants
Nancy A Cornick1, Amy F Helgerson, Volker Mai
1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50010, USA. ncornick@iastate.edu
Applied and Environmental Microbiology
|July 6, 2006
Abstract:
We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.
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