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Updated: Aug 7, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Acetate oxidation is the dominant methanogenic pathway from acetate in the absence of Methanosaetaceae
Dimitar Karakashev1, Damien J Batstone, Eric Trably
1Institute of Environment & Resources DTU, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark.
Abstract:
The oxidation of acetate to hydrogen, and the subsequent conversion of hydrogen and carbon dioxide to methane, has been regarded largely as a niche mechanism occurring at high temperatures or under inhibitory conditions. In this study, 13 anaerobic reactors and sediment from a temperate anaerobic lake were surveyed for their dominant methanogenic population by using fluorescent in situ hybridization and for the degree of acetate oxidation relative to aceticlastic conversion by using radiolabeled [2-14C]acetate in batch incubations. When Methanosaetaceae were not present, acetate oxidation was the dominant methanogenic pathway. Aceticlastic conversion was observed only in the presence of Methanosaetaceae.
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