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Updated: Jul 12, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Published on: November 26, 2008
Luminography--a new, highly sensitive visualization method for electrophoresis
R Schneppenheim1, U Budde, N Dahlmann
1Abteilung Allgemeine Paediatrie, Klinikum der Christian-Albrechts-Universität, Kiel, Germany.
Abstract:
A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.
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