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Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
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CD4+CD8+CD3high thymocytes appear transiently during ontogeny: evidence from phenotypic and functional studies

P Hugo1, R L Boyd, G A Waanders

  • 1Department of Pathology and Immunology, Monash Medical School, Parkville.

European Journal of Immunology
|November 1, 1991
PubMed

During T cell development thymocyte subsets emerge in a defined order, reflective of their maturational stage. In this study we determined the timing of appearance of CD4+CD8+CD3high thymocytes during in vivo and in vitro embryonic development, and thymic reconstitution after cortisone treatment. In these models, CD4+CD8+CD3high cells followed CD4+CD8+CD3low and preceded mature CD4+CD8-CD3high/CD4-CD8+CD3high thymocytes, while cortisone resistance was first seen among CD4+CD8+CD3high cells. CD4+CD8+CD3high thymocytes were also shown to display a pattern of antigen receptor-mediated calcium influx intermediate between that induced in other CD4+CD8+ cells and mature thymocytes. These results are consistent with a precursor-progeny relationship between CD4+CD8+CD3low and CD4+CD8+CD3high thymocytes, the latter developing to mature thymocytes (Hugo, P. et al., Int. Immunol. 1991. 3: 265).

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