Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structures related to the germ plasm in mouse.

Arkadiy Reunov1

  • 1Institute of Marine Biology, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia. arkadiy_reunov@hotmail.com

Zygote (Cambridge, England)
|July 11, 2006
PubMed
Summary

Germinal-body-like structures in mouse cells transform into nuage in spermatogonia, acting as mitochondrial cement. These structures, along with chromatoid bodies, are not found in mature spermatozoa.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Application of an Extracellular Vesicle-Based Biosensor in Early Diagnosis and Prediction of Chemoresponsiveness in Ovarian Cancer.

Cancers·2023
Same author

Predicting chemoresponsiveness in epithelial ovarian cancer patients using circulating small extracellular vesicle-derived plasma gelsolin.

Journal of ovarian research·2023
Same author

VASA-induced cytoplasmic localization of CYTB-positive mitochondrial substance occurs by destructive and nondestructive mitochondrial effusion, respectively, in early and late spermatogenic cells of the Manila clam.

Protoplasma·2021
Same author

The exosome-mediated autocrine and paracrine actions of plasma gelsolin in ovarian cancer chemoresistance.

Oncogene·2019
Same author

Close association between vasa-positive germ plasm granules and mitochondria correlates with cytoplasmic localization of 12S and 16S mtrRNAs during zebrafish spermatogenesis.

Differentiation; research in biological diversity·2019
Same author

Natural Heteroplasmy and Mitochondrial Inheritance in Bivalve Molluscs.

Integrative and comparative biology·2019

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Reproductive Biology

Background:

  • Germinal-body-like structures, nuage, and chromatoid bodies are key components of germ cells.
  • Their origin and transformation during mammalian gametogenesis are not fully understood.
  • Mitochondria play a crucial role in cellular processes and germ cell development.

Purpose of the Study:

  • To investigate the ultrastructural and morphometric characteristics of germinal-body-like structures, nuage, nuage-mitochondrial clusters, and chromatoid bodies.
  • To elucidate the transformation pathways of these structures during mouse spermatogenesis.
  • To determine the fate of these germ-plasm-related structures in mature spermatozoa.

Main Methods:

  • Ultrastructural studies using electron microscopy.
  • Morphometric analysis of cellular components.
  • Comparative analysis of germinal-body-like structures and related organelles in different stages of spermatogenesis.

Main Results:

  • Germinal-body-like structures in 4.5-day embryo cells showed no ultrastructural changes.
  • In spermatogonia, these structures were transformed into nuage, serving as 'intermitochondrial cement'.
  • Primary spermatocytes exhibited nuage-aggregated mitochondria with vacuoles containing excreted membraneous conglomerates; chromatoid bodies were not directly implicated in the germinal-body pathway and disintegrated in late spermatids, with mitochondria excreting conglomerates.

Conclusions:

  • Germinal-body-like structures undergo transformation into nuage during early spermatogenesis.
  • Nuage plays a role in organizing mitochondria in spermatogenic cells.
  • Chromatoid bodies and germinal-body-like structures follow distinct pathways, and neither is present in mature mouse spermatozoa.

Related Experiment Videos