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Updated: Aug 7, 2026

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils
Published on: April 19, 2024
Lipase-mediated conversion of vegetable oils into biodiesel using ethyl acetate as acyl acceptor
Mukesh Kumar Modi1, J R C Reddy, B V S K Rao
1Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad 500007, India.
Abstract:
Ethyl acetate was explored as an acyl acceptor for immobilized lipase-catalyzed preparation of biodiesel from the crude oils of Jatropha curcas (jatropha), Pongamia pinnata (karanj) and Helianthus annuus (sunflower). The optimum reaction conditions for interesterification of the oils with ethyl acetate were 10% of Novozym-435 (immobilized Candida antarctica lipase B) based on oil weight, ethyl acetate to oil molar ratio of 11:1 and the reaction period of 12h at 50 degrees C. The maximum yield of ethyl esters was 91.3%, 90% and 92.7% with crude jatropha, karanj and sunflower oils, respectively under the above optimum conditions. Reusability of the lipase over repeated cycles in interesterification and ethanolysis was also investigated under standard reaction conditions. The relative activity of lipase could be well maintained over twelve repeated cycles with ethyl acetate while it reached to zero by 6th cycle when ethanol was used as an acyl acceptor.
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