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Updated: Aug 7, 2026
08:06
Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
Published on: May 5, 2023
Targeting of interstitial cells using a simple gene-transfer strategy
Naohiko Fujii1, Yoshitaka Isaka, Yoshitsugu Takabatake
1Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita 565-0871, Japan.
Summary
Direct gene transfer into kidney interstitial fibroblasts is possible using parenchymal DNA injection and electroporation. This method offers a new approach for targeting these cells in tubulointerstitial fibrosis research.
Area of Science:
- Nephrology
- Molecular Biology
- Gene Therapy
Background:
- Interstitial fibroblasts play a key role in kidney inflammation and fibrosis.
- Targeting these cells is crucial for understanding and treating tubulointerstitial fibrosis.
Purpose of the Study:
- To evaluate a novel gene transfer method for interstitial cells in the kidney.
- To assess the efficiency of parenchymal DNA injection followed by electroporation.
Main Methods:
- Direct injection of DNA (oligodeoxynucleotides or expression vectors) into the kidney cortex.
- Electroporation applied to the injection site.
- Optimization of DNA amount, volume, and renal vessel clamping.
Main Results:
- Efficient gene transfer was achieved in interstitial fibroblasts, excluding tubular and glomerular cells.
- Optimal transfection occurred with 150 µg DNA and renal vessel clamping.
- Gene expression peaked at 4 days, declined by 6 weeks, but was restored by a histone deacetylase inhibitor.
Conclusions:
- Direct parenchymal injection with electroporation is an effective method for gene transfer into kidney interstitial fibroblasts.
- This technique provides a valuable tool for studying fibroblast function in renal disease.
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