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Basic fibroblast growth factor controls migration in human mesenchymal stem cells.

Annette Schmidt1, Dennis Ladage, Timo Schinköthe

  • 1Department of Molecular and Cellular Sport Medicine, German Sport University Cologne.

Stem Cells (Dayton, Ohio)
|July 11, 2006
PubMed
Summary
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Mesenchymal stem cells (MSCs) migration is crucial for tissue regeneration. Basic fibroblast growth factor (bFGF) attracts and routes MSCs by influencing their cytoskeleton and activating specific pathways.

Area of Science:

  • Stem Cell Biology
  • Cellular Migration Mechanisms
  • Tissue Engineering

Background:

  • Mesenchymal stem cells (MSCs) show therapeutic potential in tissue regeneration.
  • The migration and directional control of MSCs are critical for their efficacy but poorly understood.
  • Identifying factors that regulate MSC migration is essential for optimizing cell-based therapies.

Purpose of the Study:

  • To investigate the factors influencing mesenchymal stem cell (MSC) migration.
  • To elucidate the role of basic fibroblast growth factor (bFGF) in MSC migration and guidance.
  • To understand the signaling pathways involved in bFGF-mediated MSC migration.

Main Methods:

  • Assessing MSC migratory capability using Boyden chamber assays.
  • Evaluating the effects of various growth factors including bFGF, erythropoietin, IL-6, SDF-1β, and VEGF.

Related Experiment Videos

  • Analyzing cytoskeletal orientation and MSC behavior in response to bFGF gradients.

    • Main Results:

    • Basic fibroblast growth factor (bFGF) significantly enhanced MSC migratory activity.
    • bFGF-induced migration involved the activation of the Akt/protein kinase B (PKB) pathway.
    • MSCs exhibited directed migration and cytoskeletal alignment in response to bFGF gradients.
    • bFGF demonstrated a dual effect: attraction at low concentrations and repulsion at high concentrations.

    Conclusions:

    • bFGF plays a key role in controlling MSC migration direction and behavior.
    • The Akt/PKB pathway is involved in bFGF-mediated MSC migration.
    • The concentration-dependent ambivalent effect of bFGF allows for purposeful routing of MSCs, offering new therapeutic possibilities.