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Related Experiment Video

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A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
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A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

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Quantitative multiplexed C-reactive protein mass spectrometric immunoassay.

Urban A Kiernan1, Riccardo Addobbati, Dobrin Nedelkov

  • 1Intrinsic Bioprobes, Inc., 625 S. Smith Rd. Ste. 22, Tempe, Arizona 85281, USA. ukiernan@intrinsicbio.com

Journal of Proteome Research
|July 11, 2006
PubMed
Summary

A novel mass spectrometric immunoassay offers high sensitivity for quantifying C-reactive protein (CRP) in human plasma. This method also allows for qualitative differentiation of related proteins, showing high throughput and accuracy.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Immunology

Background:

  • C-reactive protein (CRP) is a key biomarker for inflammation.
  • Accurate quantification of CRP is crucial for clinical diagnostics.
  • Existing methods may have limitations in sensitivity or multiplexing capabilities.

Purpose of the Study:

  • To develop and validate a high-sensitivity mass spectrometric immunoassay for CRP quantification.
  • To enable simultaneous analysis of multiple plasma proteins including CRP.
  • To compare the performance of the novel assay with existing methods.

Main Methods:

  • Development of multiplexed affinity retrieval devices for simultaneous targeting of retinol binding protein, CRP, and serum amyloid P component.
  • Utilized MALDI-TOF MS for analysis with an added exogenous internal reference standard (staphylococcal enterotoxin B).
  • High-throughput analysis of standard curves, quality control, and human plasma samples.

Main Results:

  • Achieved absolute quantitative measurements for CRP with a coefficient of variation (CV) < 15%.
  • Enabled semiquantitative analysis of retinol binding protein and serum amyloid P component.
  • Successfully differentiated between CRP, retinol binding protein, serum amyloid P component, and their variants.
  • Demonstrated comparable results to a high-sensitivity latex immunoturbidimetric assay for CRP.

Conclusions:

  • The developed mass spectrometric immunoassay provides a sensitive and accurate method for CRP quantification in human plasma.
  • The multiplexing capability allows for simultaneous analysis of multiple relevant biomarkers.
  • This approach offers a robust platform for high-throughput clinical diagnostics.