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Murine central and peripheral nervous system transcriptomes: comparative gene expression.

Mark S LeDoux1, Lijing Xu, Jianfeng Xiao

  • 1University of Tennessee Health Science Center, Department of Neurology, and Center of Genomics and Bioinformatics, 855 Monroe Avenue, Link Building-Suite 415, Memphis, TN 38163, USA. mledoux@utmem.edu

Brain Research
|July 11, 2006
PubMed
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Central and peripheral nervous systems show distinct gene expression profiles. Dorsal root ganglia (DRG) are enriched in sensory perception genes, while spinal cord (SC) shows higher expression of synaptic transmission genes, aiding neuropathy research.

Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Central nervous system (CNS) and peripheral nervous system (PNS) differ significantly in development, structure, and function.
  • Neurological diseases often selectively affect specific nervous system components.
  • Understanding transcriptomic differences is key to elucidating disease mechanisms.

Purpose of the Study:

  • To investigate quantitative differences in gene expression between murine spinal cord (SC) and dorsal root ganglion (DRG).
  • To test the hypothesis of fundamental transcriptomic disparities between central and peripheral neural tissues.

Main Methods:

  • Gene expression profiling using Affymetrix GeneChip arrays on mouse SC and DRG.
  • Perfusion fixation with RNAlater to preserve RNA integrity.

Related Experiment Videos

  • Quantitative real-time RT-PCR for validation of microarray findings.

    • Main Results:

    • Identified 111 transcripts highly expressed in DRG and absent in SC, and 112 transcripts highly expressed in SC and absent in DRG.
    • DRG-enriched genes involved in G-protein coupled receptor signaling, ion transport, and sensory perception.
    • SC-enriched genes associated with synaptic transmission, neurotransmitter transport, and circulation.
    • Majority of hereditary neuropathy genes were highly enriched in DRG.

    Conclusions:

    • Significant, fundamental quantitative differences exist in neural transcriptomes between SC and DRG.
    • These distinct expression profiles offer a framework for studying SC and peripheral nervous system diseases.
    • Findings provide molecular insights into the basis of hereditary neuropathies.